Biological activity of some thiophosphamide derivatives of alkaloids with respect to influenza virus

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Drugs Exp Clin Res. 1996;22(3-5):225-8.

Biological activity of some thiophosphamide derivatives of alkaloids with respect to influenza virus.

Lisnyak OI, Lozjuk RM.

Medical Institute, Lviv, Ukraine.

Certain newly synthesized compounds belonging to the class of alkaloid ethylenimines, and those known but not subjected to the test for antiviral selection, were studied. The in vitro, in ovo and in vivo experiments detected the substances (Ukrain, amitozin, colchamine-thiotepa, thiotepa) with developed antiviral effect relative to the influenza A/H3 N2/virus strain Port Chalmers 1/73. In the work we used, 1, 10 and 100 ID50 in 0,1 ml. The virus was injected at 1, 10 and 100 EID50 per embryo. The assessment in vivo was carried out on mice of 16-18 g weight. The LD50 for mice having been determined by the probit-analysis method, therapeutic concentrations were used at 1/10 of the LD50. The preparations were found to possess an inhibitory action for the virus, along with insignificant toxicity for the systems studied. Antigrippe action was revealed by the T alkilic agent, in this case thiophosphamide, in all systems. The comparative study of the main component of the alkaloid sum, and of 2 products of their modification (amitozin and Ukrain), showed promise great for creating remedies using such methods and materials. Especially worthy of attention was the Ukrain preparation, which may be recommended as a potential antiviral remedy with the aim of widening the choice of preparations with an antiviral action.