Inhibition of angiogenesis of LCI-D20 hepatocellular carcinoma by metronomic chemotherapy of S-1

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Zhonghua Gan Zang Bing Za Zhi. 2009 Sep;17(9):665-8.

[Inhibition of angiogenesis of LCI-D20 hepatocellular carcinoma by metronomic chemotherapy of S-1.]

[Article in Chinese]
Chen FG, Wang JJ, Xue Q.
Oncology Center, the 88th Hospital of PLA, Tai'an Shandong 271000, China.
OBJECTIVE:
To investigate the role of metronomic chemotherapy of S-1 on
angiogenesis of hepatocellular carcinoma in animal model. METHODD: S-1
was dissolved in a 0.5% (w/v) HPMC solution. 30 LCI-D20 were randomly
devided into five groups: control group(O), 10 mg/kg/d S-1 group (A), 1
mg/kg/d S-1 group (B), 0.5 mg/kg/d S-1 group (C) and 0.25 mg/kg/d S-1
group (D). 28 days after the treatment with 0.5% (w/v) HPMC solution,
tumors in LCI-D20 mice were moved out. Tumor mass was measured and
microvessel density (MVD) was used to evaluate angiogenesis in tumor.
The cellular apoptosis was determined using flow cytometry. The
expression of VEGF, bFGF and TSP-1 was measured by RT-PCR. RESULTS: The
mean tumor mass was 2.01, 0.38, 1.12, 1.38, 2.27 g in O, A, B, C, D
group, respectively. The mean MVD was 39.57, 19.90, 5.93, 17.10, 29.53
in O, A, B, C, D respectively. The mean tumor cellular apoptosis rate
was 4.08%, 44.37%, 31.73%, 19.83%, and 8.25% in O, A, B, C, D
respectively. The expression of VEGF and bFGF in O group was highest,
and A was slightly low, and C and D taked the third place, and B was
the lowst; The expression of TSP-1 in B was highest, and C and D were
slightly low, and A taked the third place, and O was the lowst.
CONCLUSION: Metronomic chemotherapy of S-1 destabilizes pre-existing
tumor vasculature and inhibits ongoing angiogenesis. DOI:
10.3760/cma.j.issn.1007-3418.2009.09.007.